Abstract : The histological grading of oral submucous fibrosis has been made as grade 1,2 and 3 according to severity of fibrosis and hyalinization but the intensity of immunohistochemical staining of cytokeratin 19 has not been done to analyse and establish the fact whether the intensity increases according to histologic severity.35 cases of oral submucous fibrosis were histologically graded and immunohistochemical study was done using cytokeratin 19.Among 35 cases 8 cases of grade 1 showed 12.5% of negetive,12.5% of mild,50% of moderate and 25% of intense staining. 14 cases of Grade 2 showed 0% of negative, 64.2% of mild, 21.4% of moderate and 14.2% of intense staining. 13 cases of Grade 3 showed 0% negative, 23% mild, 46.1% moderate and 30.7% intense staining .since the overall and comparative P values were insignificant, cytokeratin 19 staining alone cannot be used as a surrogate marker to check the severity and transformation of oral submucous fibrosis to malignancy in different histologic grades of oral submucous fibrosis.